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Development of two-dimensional gel electrophoresis profiles of proteomics from GSF cells
Author(s): 
Pages: 496-499
Year: Issue:  6
Journal: Journal of Zhenjiang Medical College

Keyword:  two-dimensional gel electrophoresisGSF cell lineproteomics;
Abstract: Objective:To develop the two-dimensional gel electrophoresis(2-DE) profiles in the protemics research of GSF cell lines.Methods: GSF cells were cultured in DMEM containing 10 % fetal bovine serum,harvested after digestion by trypsin,and lysed in lysis buffer.After centrifugation,the supernatant was separated by isoelectric focusing(IEF),equilibrated and run on SDS-polyacrylamide gel electrophoresis(SDS-PAGE),and stained by silver staining followed by image scanning and analysis. Results: Higher resolvable protein spots were detected in the 2-DE profiles on the 17cm IPG strips(pH 58) of GSF cell extracts. Conclusion: The 2-DE profiles of proteomics from GSF cells were established and lay the foundation of further proteomics research of GSF cells.
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