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Expression of red fluorescent protein mediated by recombinant adeno-associated virus in bone marrow mesenchymal stem cells
Pages: 332-334
Year: Issue:  3
Journal: Qianwei Journal of Medicine & Pharmacy

Keyword:  Red fluorescent protein Recombinant adeno-associated virus Bone marrow mesenchymal stem cells;
Abstract: Objective To construct the recombinant adeno-associated virus expressing red fluorescent(DsRed)protein and investigate its transfection efficiency in vitro.Method The recombinant plasmid pAAV-DsRed was constructed by inserting the DsRed gene into the pAAV-MCS and generating the primary recombinant AAV by calcium phosphate-based technology. The transfection efficiency were evaluated by transfecting AAV-DsRed into primary rabbit BMSCs.The direction of the insertion was confirmed by PCR analysis. Results The recombinant plasmid pAAV-DsRed has been constructed successfully, and AAV-DsRed obtained;the primary BMSCs could be transfected efficiently with AAV-DsRed. Conclusion The recombinant Adeno-associated virus was constructed succeesfuly,it can transfect BMSCs and express exogenous gene, thus possess the potential for clinical application.
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