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igf-1 dui mc3t3-e1 xi bao diao wang ji diao wang diao kong dan bai bax bcl-2 biao da de ying xiang
Author(s): 
Pages: 688-689
Year: Issue:  6
Journal: Research of Traditional Chinese Medicine

Keyword:  胰岛素样生长因子-1MC3T3-E1成骨细胞无血清凋亡;
Abstract: 目的探讨胰岛素样生长因子-1(IGF-1)对小鼠前成骨样MC3T3-E1细胞凋亡基因相关蛋白Bax/Bcl-2表达的影响。方法MC3T3-E1细胞分组培养,应用4个浓度的IGF-1(1ng/mL、10ng/mL、30ng/mL、50ng/mL)干预24 h。观察MC3T3-E1细胞动态生长状况;流式细胞技术检测细胞凋亡率;免疫组织化学法检测凋亡蛋白Bax/Bcl-2的表达水平。结果与正常血清对照组相比,无血清对照组MC3T3-E1细胞凋亡率增加(P<0.05);与无血清组相比,IGF-1组MC3T3-E1细胞凋亡率降低(P<0.05),Bax表达减弱(P<0.05),Bcl-2表达增强(P<0.05);各浓度组间比较,(150)ng/mL浓度范围内MC3T3-E1细胞凋亡率随IGF-1浓度加大而逐渐降低(P<0.05),Bax表达逐渐减弱(P<0.0 5),50ng/mL浓度组Bcl-2表达明显增强(P<0.01),Bcl-2/Bax灰度比与细胞凋亡率呈显著正相关(r=0.917,P<0.01)。结论一定浓度的IGF-1同时调节Bax和Bcl-2表达,抑制无血清培养诱导的细胞凋亡。并存在剂量依赖性效应。
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