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ci er chun dui zhi fang xi bao zhi lian su mrna biao da de ying xiang
Author(s): 
Pages: 673-674
Year: Issue:  6
Journal: Research of Traditional Chinese Medicine

Keyword:  前脂肪细胞脂肪细胞雌二醇脂联素;
Abstract: 目的观察不同浓度雌二醇对原代培养人大网膜脂肪细胞脂联素mRNA表达的影响。方法体外培养人大网膜前脂肪细胞,诱导分化成熟后用雌二醇进行干预,RT-PCR方法检测脂联素mRNA水平。结果成功培养出人大网膜前脂肪细胞,雌二醇10-9mol/L10-7mol/L作用24h,不同程度地抑制脂肪细胞脂联素mRNA的表达,且有剂量依赖性。结论雌二醇可抑制脂肪细胞脂联素mRNA的表达。
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