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The Protection Effect of Beagle Dog Cerebrospinal Fluid on the Neuron
Pages: 884-886
Year: Issue:  10
Journal: Research of Traditional Chinese Medicine

Keyword:  cerebrospinal fluidneuron cultureapoptosis;
Abstract: Objective To observe the therapeutic time window of acute intracerebral hemorrhage (AICH) intervened by Beagle dog cerebrospinal fluid containing DSQ-03 herbal medicine.Methods ①The cerebral cortex neuron of SD rats were cultured by Beagle dog CSF containing the DSQ-03 in vitro.②The AICH cell models were made in vitro.③Following injury of TIH 6 h,12 h,24 h,48 h,72 h, the apoptosis of the neuron after AICH were measured by flow cytometry.Results From 0 h to 72 h after AICH,the apoptosis of the neuron appeared.The optimal effect of inhibiting the neural apoptosis was at 0 h to 24 h, and still persisted from 0 h to 72 h.Conclusion Beagle dog CSF containing DSQ-03 herbal composition was able to inhibit the neural apoptosis. The therapeutic time window of AICH treated with DSQ-03 herbal medicine was 0 h to 24 h.
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