Construction of T-vector by XcmⅠ
Author(s): WU Guoqiu, SHEN Zilong
Pages: 293-295
Year: 2002 Issue: 4
Journal: Journal of Nanjing Railway Medical College
Keyword: T-vector; construction; XcmⅠ;
Abstract: Objective To construct T-vector with characteristics of replicating itself.Methods By designing two primers on the site of XcmⅠ,550bp fragment was amplified by polymerase chain reaction(PCR) using template of PET28(a) +;the plasmid with inserted fragment was identified by methods of restrictive analysis,PCR and DNA sequencing.Results Two bands of 540bp and 3810bp appeared by XcmⅠ;550bp fragment can be amplified by PCR;and sequencing result was in accordance with the expected sequence.Conclusion A circular vector with two XcmⅠ sites has been successfully constructed.
Pages: 293-295
Year: 2002 Issue: 4
Journal: Journal of Nanjing Railway Medical College
Keyword: T-vector; construction; XcmⅠ;
Abstract: Objective To construct T-vector with characteristics of replicating itself.Methods By designing two primers on the site of XcmⅠ,550bp fragment was amplified by polymerase chain reaction(PCR) using template of PET28(a) +;the plasmid with inserted fragment was identified by methods of restrictive analysis,PCR and DNA sequencing.Results Two bands of 540bp and 3810bp appeared by XcmⅠ;550bp fragment can be amplified by PCR;and sequencing result was in accordance with the expected sequence.Conclusion A circular vector with two XcmⅠ sites has been successfully constructed.
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