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Issue:
Isolation of Microsatellite Fragments in Prunus salicina Using Modified SAM Method
Author(s):
ZHANG Huirong
,
XUE Huabai
,
QIAO Yushan
,
CAO Shangyin
,
ZHANG Jianwei
Pages:
90
-
93
Year:
2009
Issue:
3
Journal:
Nonwood Forest Research
Abstract:
对选择性扩增微卫星法进行了改进,应用于分离中国李中的微卫星,共分离出17个中国李特异微卫星位点,相应设计了17条引物.并分别与对应的5\'锚定简并微卫星引物配对应用于5个中国李品种的基因组PCR扩增.结果表明:除PCA04、PCA07、PCA11 3条引物没有扩增产物外,其余14条引物在5个受试中国李品种中均有扩增产物.
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