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hplc-uv ni he zi wai tu pu shi bie huang zuo zhong huang tong you xiao bu wei ji qi han liang ce ding
Author(s): 
Pages: 266-267
Year: Issue:  3
Journal: Journal of Liaoning College of Traditional Chinese

Keyword:  黄芩黄酮HPLC含量测定;
Abstract: 目的:探讨黄芩中总黄酮的识别和含量测定方法。方法:在不同的测定波长下,采用HPLC法绘制黄芩色谱流出曲线,然后描绘每个色谱峰的峰面积-波长曲线,拟合紫外图谱,识别黄酮有效部位,进而进行含量测定。结果:总黄酮以黄芩苷计,有效部位的含量分别为14.6%、15.4%、15.2%。结论:HPLC-UV法可作为黄芩中总黄酮识别和含量测定的方法。
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