Effects of isoflurane and enflurane on spontaneous neural discharge of hippocampus in rats
Author(s): ZHAO Xinjing, XU Lixian, YE Pingan
Pages: 58-60
Year: 2004 Issue: 1
Journal: Journal of Xi'an Medical University(Chinese)
Keyword: isoflurane; enflurane; hippocampus; brain slice; patchclamp;
Abstract: Objective To investigate the effects of inhaling isoflurane and enflurane on the spontaneous neural discharge of the neurones in rat hippocampus. Methods Whole cell patchclamp recording te chnique was used to observe the effects of isoflurane and enflurane on the spont aneous discharge rate of the neurons in the hippocampus on the brain slice of ne w-born SD rats. After decapitation, the whole brain of the rat was removed and put into artificial cerebrospinal fluid (ACSF) saturated with 1.36 g·L -1 O 2 and 0.098 g·L -1 CO 2 mixed gas at 4 ℃ . Brain was cut into 300～400 μ m thick slices containing the hippocampus. Whole cell patchc lamp recording technique was used to observe the effects of isoflurane with dif ferent concentrations on the spontaneous discharge rate of neurons in the hippoc ampus on the brain slices. Results Isoflurane and enfluran e could significantly inhibit the spontaneous neural discharge of neurons in the hippocampus in a dose-dependant manner. The effects of spontaneous neural disc harge of hippocampus inhibited by isoflurane (0.12 g·L -1 ～0.36 g·L -1 ) and enflurane (0.2 g·L -1 ～0.6 g·L -1 ) could be recove red following washing off with ACSF for 5 min. Conclusion T he spontaneous discharge rate of neurons in the hippocampus can be reversibly in hibited by isoflurane and enflurane. Hippocampus may be an important action site of anesthetics isoflurane and enflurane in the central nervous system.
Pages: 58-60
Year: 2004 Issue: 1
Journal: Journal of Xi'an Medical University(Chinese)
Keyword: isoflurane; enflurane; hippocampus; brain slice; patchclamp;
Abstract: Objective To investigate the effects of inhaling isoflurane and enflurane on the spontaneous neural discharge of the neurones in rat hippocampus. Methods Whole cell patchclamp recording te chnique was used to observe the effects of isoflurane and enflurane on the spont aneous discharge rate of the neurons in the hippocampus on the brain slice of ne w-born SD rats. After decapitation, the whole brain of the rat was removed and put into artificial cerebrospinal fluid (ACSF) saturated with 1.36 g·L -1 O 2 and 0.098 g·L -1 CO 2 mixed gas at 4 ℃ . Brain was cut into 300～400 μ m thick slices containing the hippocampus. Whole cell patchc lamp recording technique was used to observe the effects of isoflurane with dif ferent concentrations on the spontaneous discharge rate of neurons in the hippoc ampus on the brain slices. Results Isoflurane and enfluran e could significantly inhibit the spontaneous neural discharge of neurons in the hippocampus in a dose-dependant manner. The effects of spontaneous neural disc harge of hippocampus inhibited by isoflurane (0.12 g·L -1 ～0.36 g·L -1 ) and enflurane (0.2 g·L -1 ～0.6 g·L -1 ) could be recove red following washing off with ACSF for 5 min. Conclusion T he spontaneous discharge rate of neurons in the hippocampus can be reversibly in hibited by isoflurane and enflurane. Hippocampus may be an important action site of anesthetics isoflurane and enflurane in the central nervous system.
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