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Differentially expressed gene analysis of populus induced by Tetra lobulifera(Keifer)
Author(s): 
Pages: 8-12
Year: Issue:  4
Journal: Journal of Nanjing Forestry University(Natural Science Edition)

Keyword:  杨四瘿螨RT-PCRRACEPtWRKY;
Abstract: 采用抑制性消减杂交(suppression subtractive hybridization,SSH)技术,在构建杨四瘿螨诱导差异表达的抑制消减cDNA文库的基础上,通过RT-PCR技术对候选基因进行了验证,其中有6条差异表达候选基因在4个时间点表达量有明显的增强.运用RACE(Rapid Amplification of cDNA Ends)技术克隆获得了一段全长2 053 bp的cDNA克隆...
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