Effectsof2, 3-Dioxoindolineon Anti-proliferation and Apoptosis n MCF-7 and HT-29 Cell Lines
Author(s): WANG Yanli, HOU Lin, SONG Jinlian, GE Yinlin, YUE Wang
Pages: 824-828
Year: 2009 Issue: 5
Journal: Biomagnetism
Keyword: 2; 3Dioxoindoline; MCF-7; HT-29; Bcl-2; Apoptosis;
Abstract: Objective: To investigate the effects of 2,3-dioxoindoline (ISA) on proliferation and apoptosis in human breast cancer cell(MCF-7) and human colon cancer cell(HT-29). Methods: MTT assay was employed to examine the antiproliferative effect of ISA(50, 100, 200, 400 μmol·L-1)for various time periods; the two cell lines treated with 400 μmol·L-1 ISA for 48 h were stained with Hochest33258. Changes of nucleolus were observed under fluorescent microscope. The expression levels of protein Bcl-2 and Bax were analyzed by Western Blotting. The levels of bcl-2 mRNA and bax mRNA were examined by RT-PCR. Results: ISA could inhibit the proliferation of the both cell lines obviously within a certain concentration. ②Treated with 400 μmol·L-1 ISA for 48 h, the typical apoptotic morphologic changes could be observed, including chromatin margination, nuclear condensation and apoptotic bodies. ③Compared with that of the control, the expressions of bcl-2 mRNA and protein were decreased significantly in a dose-dependent manner (P<0.01 or P<0.05). However, bax mRNA and protein expression had no obvious change (P>0.05). Conclusions: ISA could inhibit the proliferation and further induce the apoptosis of the cell lines. Its mechanism may be related with the down-regulation of the expression of bcl-2.
Pages: 824-828
Year: 2009 Issue: 5
Journal: Biomagnetism
Keyword: 2; 3Dioxoindoline; MCF-7; HT-29; Bcl-2; Apoptosis;
Abstract: Objective: To investigate the effects of 2,3-dioxoindoline (ISA) on proliferation and apoptosis in human breast cancer cell(MCF-7) and human colon cancer cell(HT-29). Methods: MTT assay was employed to examine the antiproliferative effect of ISA(50, 100, 200, 400 μmol·L-1)for various time periods; the two cell lines treated with 400 μmol·L-1 ISA for 48 h were stained with Hochest33258. Changes of nucleolus were observed under fluorescent microscope. The expression levels of protein Bcl-2 and Bax were analyzed by Western Blotting. The levels of bcl-2 mRNA and bax mRNA were examined by RT-PCR. Results: ISA could inhibit the proliferation of the both cell lines obviously within a certain concentration. ②Treated with 400 μmol·L-1 ISA for 48 h, the typical apoptotic morphologic changes could be observed, including chromatin margination, nuclear condensation and apoptotic bodies. ③Compared with that of the control, the expressions of bcl-2 mRNA and protein were decreased significantly in a dose-dependent manner (P<0.01 or P<0.05). However, bax mRNA and protein expression had no obvious change (P>0.05). Conclusions: ISA could inhibit the proliferation and further induce the apoptosis of the cell lines. Its mechanism may be related with the down-regulation of the expression of bcl-2.
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