Determination of survivin-3 in human hepatoma HepG2 cells and construction of its eukaryotic expression vector
Author(s): XU Bing, YANG Laihua
Pages: 1075-1077
Year: 2008 Issue: 6
Journal: Biomagnetism
Keyword: SVV-3; Eukaryotic expression vector; hepatoma HepG2;
Abstract: Objective To determine survivin(SVV)variant in human hepatoma cell line-HepG2 and to construct its eukaryotic expression vector.Methods The total RNA of HepG2 was extracted;3 special primers were designed and identified;SVV-3 primers containing double restriction cndonuclease BamHⅠand XhoⅠwere designed;the whole coding area of SVV-3 was amplified by RT-PCR;the amplified product was double-digested by BamHⅠand XhoⅠand cloned into pcDNA3.1,detecting its sequence.Results SVV-3 was expressed in HepG2 more than SVV-1.The sequence is consistent with SVV-3 sequence published in Gen-Bank. Conclusions SVV-1 and SVV-3 were detected to be expressed in HepG2.A recombinant plasmid vector containing human SVV-3 was constructed successfully.
Pages: 1075-1077
Year: 2008 Issue: 6
Journal: Biomagnetism
Keyword: SVV-3; Eukaryotic expression vector; hepatoma HepG2;
Abstract: Objective To determine survivin(SVV)variant in human hepatoma cell line-HepG2 and to construct its eukaryotic expression vector.Methods The total RNA of HepG2 was extracted;3 special primers were designed and identified;SVV-3 primers containing double restriction cndonuclease BamHⅠand XhoⅠwere designed;the whole coding area of SVV-3 was amplified by RT-PCR;the amplified product was double-digested by BamHⅠand XhoⅠand cloned into pcDNA3.1,detecting its sequence.Results SVV-3 was expressed in HepG2 more than SVV-1.The sequence is consistent with SVV-3 sequence published in Gen-Bank. Conclusions SVV-1 and SVV-3 were detected to be expressed in HepG2.A recombinant plasmid vector containing human SVV-3 was constructed successfully.
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