A Study of Andrographitis to Prevent Bacterial Translocation in a Rat Model of Experimental Cirrhosis
Author(s): WU Chunming, ZUO Huimin, LI Hongtao, SONG Jianxin
Pages: 596-599
Year: 2007 Issue: 5
Journal: Acta Universitatis Medicinae Tongji
Keyword: liver cirrhosis; andrographitis; bacterial translocation;
Abstract: Objective To evaluate the effects of andrographitis on bacterial translocation in experimental cirrhotic rats.Methods Thirty rats with CCl4-induced cirrhosis were randomly divided into 4 groups:group AN [n=8,andrographitis 0.5 g/(kg·d)],group NF [n=8,norfloxin 0.3 g/(kg·d)],group AN+NF [n=8,androgtaphitis 0.5 g/(kg·d)+norfloxacin 0.3 g/(kg·d)],group CN [n=6,0.9% normal saline 2 ml/(kg·d)].After treatment for 2 weeks,the mortality of each group was analyzed.Blood specimens were collected from peripheral vein for detections of serum levels of ALT,AST,TNF-α and NO.The MLNs,blood,liver,spleen and contents of ileum were obtained and the samples were cultured.Intestinal permeability was measured by HPLC with RID.Parts of liver and ileum were cut into sections for pathological examinations.Results Compared with group CN,the serum levels of TNF-α and NO in group AN,group NF and group AN+NF were decreased significantly(P<0.05) and the intestinal permeability in group AN and group NF was also decreased remarkably(P<0.05).Total ileum bacterial counts and incidence of bacterial translocation in group CN were significantly higher than in other groups(P<0.05).The ileum histological appearance in group CN was obviously abnormal,and villi were shortened and damaged with submucosal inflammatory infiltration.Conclusion Andrographitis can effectively prevent cirrhotic rats from developing bacterial translocation in intestinal tract.
Pages: 596-599
Year: 2007 Issue: 5
Journal: Acta Universitatis Medicinae Tongji
Keyword: liver cirrhosis; andrographitis; bacterial translocation;
Abstract: Objective To evaluate the effects of andrographitis on bacterial translocation in experimental cirrhotic rats.Methods Thirty rats with CCl4-induced cirrhosis were randomly divided into 4 groups:group AN [n=8,andrographitis 0.5 g/(kg·d)],group NF [n=8,norfloxin 0.3 g/(kg·d)],group AN+NF [n=8,androgtaphitis 0.5 g/(kg·d)+norfloxacin 0.3 g/(kg·d)],group CN [n=6,0.9% normal saline 2 ml/(kg·d)].After treatment for 2 weeks,the mortality of each group was analyzed.Blood specimens were collected from peripheral vein for detections of serum levels of ALT,AST,TNF-α and NO.The MLNs,blood,liver,spleen and contents of ileum were obtained and the samples were cultured.Intestinal permeability was measured by HPLC with RID.Parts of liver and ileum were cut into sections for pathological examinations.Results Compared with group CN,the serum levels of TNF-α and NO in group AN,group NF and group AN+NF were decreased significantly(P<0.05) and the intestinal permeability in group AN and group NF was also decreased remarkably(P<0.05).Total ileum bacterial counts and incidence of bacterial translocation in group CN were significantly higher than in other groups(P<0.05).The ileum histological appearance in group CN was obviously abnormal,and villi were shortened and damaged with submucosal inflammatory infiltration.Conclusion Andrographitis can effectively prevent cirrhotic rats from developing bacterial translocation in intestinal tract.
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