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Morphometric Analysis of Spinal Motoneurons
Author(s): ZHAO Heqing, YANG Xiaoling, LI Wen
Pages: 87-
88,101
Year: 2005
Issue:
5
Journal: Acta Academiae Medicinae Suzhou
Keyword: spinal motoneurons; morphometric;
Abstract: Objective This paper is to grasp the survival rate and the morphometric properities of motoneurons at different culturing time,so we understand their growing regulations.We know(what's) time to add experimental factors and what's time to get outcome according to these findings.Methods The number of spinal motoneurons was counted at different culturing time(such as 0;1;3;5;7;9days)under microscope at250×.ChAT-positive neurons were chosen as analysized subjects.Five indexes such as axonal length,total dendrites,number of bifurcation,dendrites and cell body area were measured using image analysis system. Results Survival rate of spinal motoneurons trended to decrease with time passing by.It dropped to 59% for the first day and maintained at the rate of 50% following three to five days.The survival dropped gradually to 37% at the seventh day.Most of neurons had been dead after nine days.When it concerned to morphology,not only axonal length,dendrites length,dendrites number but also cell body area increased rapidly for the first three days,and improved steadily from the fourth day to the seventh day,then decreased sharply after culturing seven days.All neurons were going to die with body withering and dendrites rupturing at the ninth day.Conclusions We can observe that the growing condition of spinal motoneurons through these following indexes: survival rate,axonal length,dendrites length,dendrites number,the number of bifurcation and cell body area.Neurons growing from the third day to the fifth day can be chosen to measure their morphometric indexes because motoneurons have grown best in this period.
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