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Rapid Screening of Nitrilase Producing Strains from Soil
Author(s): 
Pages: 545-548
Year: Issue:  4
Journal: Journal of East China University of Science and Technology(Natural Science Edition)

Keyword:  腈水解酶苯酚-次氯酸盐比色法(Berthelot比色法)微孔板筛选;
Abstract: 为了从土壤中快速筛选具有腈水解能力的微生物,建立了以苯酚一次氯酸盐比色法(又名Berthelot比色法)检测96孔板反应产物中氨离子浓度为基础的腈水解酶酶活快速分析方法.用该法从土壤样本中筛选获得了9个酶活力较高的菌株.用HPLC方法定量检测9个菌株对对羟基苯甲腈的转化率,证明苯酚-次氯酸盐比色法微孔板分光光度计的测定结果与HPLC结果对应良好,转化率最高为49.9%.经16s rDNA鉴定,菌株...
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