influence of preparation conditions on properties of chemical modified nylon affinity membrane (used for -globulin adsorption)
Author(s): GAN Hongyu, SHANG Zhenhua, WANG Junde, LIU Xueliang
Pages: 516-520
Year: 2000 Issue: 4
Journal: Chinese Journal of Chemistry
Keyword: Preparation; property; affinity chromatography; nylon membrane; γ-globulin;
Abstract: Microporous polyamide membrane (Nylon 6, PA) was modified by add hydrolysis and then coupled with hydroxyethyl cellulose (HEC) to amplify reactive groups and reduce nonspecific interaction with proteins. 1,6-Diaminohexane (DAH) as space arm and phenylalanine (Phe) as ligand were immobilized onto nylon membrane through the activation of s-triazine trichloride (CyCl3). The affinity membrane obtained can be used to adsorb γ-globulin specifically. The optimum conditions of preparing affinity membrane and adsorbing bovine γ-globulin were studied.
Pages: 516-520
Year: 2000 Issue: 4
Journal: Chinese Journal of Chemistry
Keyword: Preparation; property; affinity chromatography; nylon membrane; γ-globulin;
Abstract: Microporous polyamide membrane (Nylon 6, PA) was modified by add hydrolysis and then coupled with hydroxyethyl cellulose (HEC) to amplify reactive groups and reduce nonspecific interaction with proteins. 1,6-Diaminohexane (DAH) as space arm and phenylalanine (Phe) as ligand were immobilized onto nylon membrane through the activation of s-triazine trichloride (CyCl3). The affinity membrane obtained can be used to adsorb γ-globulin specifically. The optimum conditions of preparing affinity membrane and adsorbing bovine γ-globulin were studied.
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