Improvement of Prepared Method for Tetanus Antitoxin
Author(s): YAN Shu-qin, XUE Xiang-guang, LIU Chun-hong
Pages: 43-45
Year: 2004 Issue: 3
Journal: The Chinese Academic Medical Magazine of Organisms
Keyword: Fragment F(ab’)2 ; Titer ; Stability;
Abstract: To improve the prepared method of tetanus antitioxin,in order to increase the content of fragment F (ab')2.Method Immuned horses as routine pathway, Treated horses blood plasma by enhancing gastric enzyme digestion time ,adjusting pepsinogen ratio and pH,then further purify by ammonium sulfate precipitation,alum adsorption,ultrafiltration and degerming,The content of F (ab')2 was detected by electrophoresis.Results As above production condition , The ratio content of F (ab')2 to total protein was 60% up to 90% .Conclusion The method was suitable for increasing the content of F (ab')2 of tetanus antitoxin .
Pages: 43-45
Year: 2004 Issue: 3
Journal: The Chinese Academic Medical Magazine of Organisms
Keyword: Fragment F(ab’)2 ; Titer ; Stability;
Abstract: To improve the prepared method of tetanus antitioxin,in order to increase the content of fragment F (ab')2.Method Immuned horses as routine pathway, Treated horses blood plasma by enhancing gastric enzyme digestion time ,adjusting pepsinogen ratio and pH,then further purify by ammonium sulfate precipitation,alum adsorption,ultrafiltration and degerming,The content of F (ab')2 was detected by electrophoresis.Results As above production condition , The ratio content of F (ab')2 to total protein was 60% up to 90% .Conclusion The method was suitable for increasing the content of F (ab')2 of tetanus antitoxin .
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