Interleukin-18 expression in peripheral blood mononuclear cells in children with steroid-resistant nephrotic syndrome
Author(s): JIANG Hong, LUO Gang, JIANG Hong
Pages: 337-340
Year: 2009 Issue: 5
Journal: Chinese Journal of Contemporary Pediatrics
Keyword: Nephrotic syndrome; Interleukin-18; Steroid-resistance; Child;
Abstract: Objective To examine serum concentration of interleukin-18 (IL-18) and IL-18 mRNA expression in peripheral blood mononuclear cells (PBMCs) in children with primary nephrotic syndrome (PNS) and explore the possible role of IL-18 in steroid-resistant nephrotic syndrome (SRNS).Methods Sixty-six children with newly diagnosed PNS,including 39 cases of steroid sensitive nephrotic syndrome (SSNS) and 27 cases of SRNS,were enrolled. Forty healthy children were used as a normal control group. Blood samples were collected before and 8 weeks after glucocorticoid treatment. Serum concentration of IL-18 was measured using ELISA. IL-18 mRNA expression in PBMCs was detected by the RT-PCR method. The amount of 24-hr urine protein was measured by the biuret method. Serum contents of total cholesterol (T-Ch),triglyceride (TG),low density lipoprotein (LDL),total protein (TP),and albumin (Alb) were measured by the automatic biochemistry analyzer.Results Serum concentration of IL-18 and IL-18 mRNA expression in PBMCs in the SSNS and the SRNS groups were significantly higher than those in the normal control group before treatment (P<0.05). The SRNS group had increased serum protein concentration of IL-18 and IL-18 mRNA expression in PBMCs compared with the SSNS group before treatment (P<0.05). Serum LDL content in the SRNS group was also significantly higher than that in the SSNS group before treatment (P<0.05). After treatment,serum concentration of IL-18 and IL-18 mRNA expression in PBMCs in the SRNS group were significantly higher than those in the SSNS and the normal control groups (P<0.05). Serum concentration of IL-18 and IL-18 mRNA expression in PBMCs in the SSNS group were significantly reduced after treatment,but the alterations of IL-18 were not observed in the SRNS group after treatment.Conclusions SRNS was associated with increased serum IL-18 concentration and IL-18 mRNA expression in PBMCs. Over-production of IL-18 may play a role in the development of SRNS.
Pages: 337-340
Year: 2009 Issue: 5
Journal: Chinese Journal of Contemporary Pediatrics
Keyword: Nephrotic syndrome; Interleukin-18; Steroid-resistance; Child;
Abstract: Objective To examine serum concentration of interleukin-18 (IL-18) and IL-18 mRNA expression in peripheral blood mononuclear cells (PBMCs) in children with primary nephrotic syndrome (PNS) and explore the possible role of IL-18 in steroid-resistant nephrotic syndrome (SRNS).Methods Sixty-six children with newly diagnosed PNS,including 39 cases of steroid sensitive nephrotic syndrome (SSNS) and 27 cases of SRNS,were enrolled. Forty healthy children were used as a normal control group. Blood samples were collected before and 8 weeks after glucocorticoid treatment. Serum concentration of IL-18 was measured using ELISA. IL-18 mRNA expression in PBMCs was detected by the RT-PCR method. The amount of 24-hr urine protein was measured by the biuret method. Serum contents of total cholesterol (T-Ch),triglyceride (TG),low density lipoprotein (LDL),total protein (TP),and albumin (Alb) were measured by the automatic biochemistry analyzer.Results Serum concentration of IL-18 and IL-18 mRNA expression in PBMCs in the SSNS and the SRNS groups were significantly higher than those in the normal control group before treatment (P<0.05). The SRNS group had increased serum protein concentration of IL-18 and IL-18 mRNA expression in PBMCs compared with the SSNS group before treatment (P<0.05). Serum LDL content in the SRNS group was also significantly higher than that in the SSNS group before treatment (P<0.05). After treatment,serum concentration of IL-18 and IL-18 mRNA expression in PBMCs in the SRNS group were significantly higher than those in the SSNS and the normal control groups (P<0.05). Serum concentration of IL-18 and IL-18 mRNA expression in PBMCs in the SSNS group were significantly reduced after treatment,but the alterations of IL-18 were not observed in the SRNS group after treatment.Conclusions SRNS was associated with increased serum IL-18 concentration and IL-18 mRNA expression in PBMCs. Over-production of IL-18 may play a role in the development of SRNS.
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