Study on excretion of ginsenoside Rg_2 in rats
Author(s): YANG Xiuwei, GUI Fangjin, SONG Yan, ZHANG Weiqing, TIAN Jianming, LI Longyun
Pages: 1281-1284
Year: 2009 Issue: 10
Journal: China Journal of Chinese Materia Medica
Keyword: ginsenoside Rg2; drug excretion; high-performance liquid chromatography;
Abstract: Objective:To determine the ginsenoside Rg2 and study its excretion in bile,feces and urine of rat.Method: Reversed phase high-performance liquid chromatographic(RP-HPLC) method with an ultra-violet detector(UVD) was performed at a detection wavelength of 203 nm and with a Dikma DiamonsilTM C18 column(4.6 mm×250 mm,5μm),which the mobile phase was consisted of methanol-aq.4% H3PO4(65∶35),for determination of the ginsenoside Rg2 in bile,feces and urine after administration of the ginsenoside Rg2 to rat at a tail vein single dose of 20 mg·kg-1.Result: The HPLC-UVD method fulfilled all the standard requirements of linearity,recovery,precision,and accuracy.After tail vein administration of the ginsenoside Rg2 to rat,the 5.5 hour cumulative biliary excretion rate and the 24-hour cumulative feces excretion rate of intact ginsenoside Rg2 were 27.2% and 22.6% of the administered dose,respectively.But intact ginsenoside Rg2 could not be detected in urine during this experimental period.Conclusion: The bile and feces were the main excretion routs of the unchanged form after tail vein administration of the ginsenoside Rg2 to rat.
Pages: 1281-1284
Year: 2009 Issue: 10
Journal: China Journal of Chinese Materia Medica
Keyword: ginsenoside Rg2; drug excretion; high-performance liquid chromatography;
Abstract: Objective:To determine the ginsenoside Rg2 and study its excretion in bile,feces and urine of rat.Method: Reversed phase high-performance liquid chromatographic(RP-HPLC) method with an ultra-violet detector(UVD) was performed at a detection wavelength of 203 nm and with a Dikma DiamonsilTM C18 column(4.6 mm×250 mm,5μm),which the mobile phase was consisted of methanol-aq.4% H3PO4(65∶35),for determination of the ginsenoside Rg2 in bile,feces and urine after administration of the ginsenoside Rg2 to rat at a tail vein single dose of 20 mg·kg-1.Result: The HPLC-UVD method fulfilled all the standard requirements of linearity,recovery,precision,and accuracy.After tail vein administration of the ginsenoside Rg2 to rat,the 5.5 hour cumulative biliary excretion rate and the 24-hour cumulative feces excretion rate of intact ginsenoside Rg2 were 27.2% and 22.6% of the administered dose,respectively.But intact ginsenoside Rg2 could not be detected in urine during this experimental period.Conclusion: The bile and feces were the main excretion routs of the unchanged form after tail vein administration of the ginsenoside Rg2 to rat.
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