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Clone and expression of Sonic Hedgehog (Shh) gene from embryonic mice skin
Author(s): 
Pages: 665-667
Year: Issue:  9
Journal: China journal of Leprosy and Skin Diseases

Keyword:  Sonic Hedgehog (Shh)克隆基因表达ICR鼠;
Abstract: 目的:检测ICR胎鼠皮肤组织克隆Sonic Hedgehog(Shh)基因在胎鼠皮肤的表达.方法:分离12.5~16.5 d胎龄的胎鼠背部皮肤,用Trizol-酚-氯仿抽提法提取总RNA,应用RT-PCR技术扩增胎鼠皮肤Shh全部编码基因,重组于pMD19-T载体进行克隆测序分析.应用合成地高辛标记的反义Shh-mRNA探针,整体原位杂交技术检测Shh基因在ICR鼠皮肤上的表达.结果:从15.5 d胎鼠背部皮肤中成功扩增Shh基因,Blast分析与GeneBank公布的Shh基因编码区一致.同时15.5 d以后胎鼠背部皮肤检测到Shh基因的表达.结论:Shh基因可能参与了皮肤毛囊的形成.
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