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Influence of different cultural conditions on germ tube formation of Candida albicans
Author(s): 
Pages: 651-653
Year: Issue:  9
Journal: China journal of Leprosy and Skin Diseases

Keyword:  白念珠菌芽管结晶紫染色法;
Abstract: 目的:比较不同培养条件对白念珠菌芽管形成的影响.方法:分别采用胎牛血清(FCS)、灭活人血清、RPMI1640培养基、DMEM培养基,在不同条件(孵育温度、孵育时长、pH值、起始菌浓度)下进行芽管的诱导培养,并采用结晶紫染色法计算出芽率.结果:相同条件下,FCS比RPMI1640、DMEM培养基诱导出芽率高(P<0.05),而后两者之间无显著性差异(P>0.05);白念珠菌起始菌体浓度为5×106 cfu/mL时,于FCS中37℃振荡孵育2 h,出芽率及芽管纯度最好;当菌体浓度超过5×106 cfu/mL时,随着菌体浓度的增加,出芽率反而下降.结论:优化培养条件可获得较纯的芽管,结晶紫染色法可精确定量检测芽管形成率.
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