Localization and characterization of Fe(Ⅲ) reductase of Desulfovibrio dechloracetivorans strain SF3
Author(s): WANG Jia-chen, LIN Yang, SUN Bao-lin
Pages: 337-343
Year: 2009 Issue: 4
Journal: Journal of University of Science and Technology of China
Keyword: Fe(Ⅲ) reductase; Desulfovibrio dechloracetivorans strain SF3; localization; characterization;
Abstract: Both physiological and biochemical studies have demonstrate that Fe(Ⅲ)-EDTA is a favorite soluble Fe(Ⅲ) of strain SF3. The UV-visible spectra of dithionite-reduced, soluble Fe(Ⅲ) reoxidized cell extracts indicates the involvement of c-type cytochromes in Fe(Ⅲ) reduction. Fractionation experiments suggest that 87% of the Fe(Ⅲ) reducing activity is localized at soluble fraction, 74% of the Fe(Ⅲ)-reducing activity is found in the spheroplasts, which may indicate a distinct mechanism for Fe(Ⅲ) reduction. Fe(Ⅲ) reductase of strain SF3 exhibits the highest activity at 25 ℃, pH 7 4, and shows a great oxygen-tolerance ability.
Pages: 337-343
Year: 2009 Issue: 4
Journal: Journal of University of Science and Technology of China
Keyword: Fe(Ⅲ) reductase; Desulfovibrio dechloracetivorans strain SF3; localization; characterization;
Abstract: Both physiological and biochemical studies have demonstrate that Fe(Ⅲ)-EDTA is a favorite soluble Fe(Ⅲ) of strain SF3. The UV-visible spectra of dithionite-reduced, soluble Fe(Ⅲ) reoxidized cell extracts indicates the involvement of c-type cytochromes in Fe(Ⅲ) reduction. Fractionation experiments suggest that 87% of the Fe(Ⅲ) reducing activity is localized at soluble fraction, 74% of the Fe(Ⅲ)-reducing activity is found in the spheroplasts, which may indicate a distinct mechanism for Fe(Ⅲ) reduction. Fe(Ⅲ) reductase of strain SF3 exhibits the highest activity at 25 ℃, pH 7 4, and shows a great oxygen-tolerance ability.
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