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Metabolic flux analysis of L-ornithine biosynthesis by Corynebacterium glutamicum
Author(s): 
Pages: 36-40
Year: Issue:  4
Journal: Journal of Nanjing University of Technology(Natural Science Edition)

Keyword:  代谢流分析L-鸟氨酸谷氨酸棒杆菌;
Abstract: 应用代谢流平衡模型,通过物料衡算和Matlab线性规划的方法得到谷氨酸棒杆菌1009生物合成L-鸟氨酸的代谢流分布与理想代谢流分布.结果表明:在分批培养条件下,生物合成L-鸟氨酸的过程中,有58.6%的C架进入糖酵解途径,41.4%的C架进入戊糖磷酸途径,L-鸟氨酸的产量达到理论最大产率的65.4%;与理论值相比,应降低三羧酸循环的代谢流,同时减少副产物氨基酸和有机酸的生成.
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