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Studies on the HPLC Fingerprint Spectrum of Flavonoids in Extract of Dendranthema indicum
Author(s): 
Pages: 2941-2943
Year: Issue:  12
Journal: Lishizhen Medicine and Materia Medica Research

Keyword:  神农香菊提取物高效液相色谱指纹图谱;
Abstract: 目的 建立神农香菊提取物黄酮类成分高效液相色谱(HPLC)指纹图谱.方法 采用反相高效液相色谱法,色谱柱为DIONEX-ACCLAIM.120 C18(250 mm×4.6 mm,5 μm);流动相为磷酸盐缓冲溶液(pH=2.5)-甲醇(梯度洗脱);流速为0.8 ml/min;柱温35℃;检测波长350 nm.结果 建立了神农香菊提取物黄酮类成分HPLC指纹图谱,标定了16个共有指纹特征峰,方法学考察符合指纹图谱技术要求.结论 方法稳定,可靠,精密度高,重复性好,可为神农香菊提取物质量标准的制定提供科学依据.
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