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Determination of Tetrahydropalmatine in Compound Yuanhuzhitong Patch by HPLC
Author(s): Han Jianwei, Zhang Li, Gao Xiaochun
Pages: 621-
623
Year: 2009
Issue:
5
Journal: China Pharmacist
Keyword: 高效液相色谱法; 含量测定; 复方元胡止痛膏贴; 延胡索乙素;
Abstract: 目的:建立高效液相色谱法测定复方元胡止痛膏贴中延胡索乙素含量的方法.方法:采用Zorbax SB-C18色谱柱(250mm×4.6mm,5μm);以甲醇-0.1%磷酸溶液(三乙胺调pH至6.0)(64:36)为流动相;流速0.8ml·min-1;检测波长280nm.结果:延胡索乙素线性范围0.11~1.15μg(r=0.9999),平均回收率和RSD分别为96.7%和2.4%.结论:方法简便、可靠,可用于复方元胡止痛膏贴中延胡索乙素含量测定.
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