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Cloning of CAST partial cDNA sequence in Jiulong yak and its tissue differential expression
Pages: 764-767
Year: Issue:  4
Journal: Journal of Southwest University for Nationalities(Natrual Science Edition)

Keyword:  Jiulong yakCAST geneclonetissue expression ;
Abstract: A pair of primers is designed according to bovine CAST sequence. Total RNA is extracted from adult jiulong-yak. 485bp of Jiulong-yak CAST gene are obtained by RT-PCR method and GenBank number is FJ483833. It is found that CAST gene of Jiulong-yak has 99 % homolog with cattle, using DNAman software. Then the specific primers are designed according to the CAST sequence obtained. SQ RT-PCR method is used to detect the mRNA differential expression of CAST in tissues. The results show CAST expressed in adipose tissue, muscle, liver, kidney and spleen, but expression of CAST in adipose tissue is significant lower than other tissues (P<0.01).
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