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Study on Medium of Tissue Culture of Yabukita
Author(s): Yang yanlei, Liu chaowei
Pages: 101-
102
Year: 2008
Issue:
22
Journal: Auhui Agricultural Science Bulletin
Keyword: 菽北种茶; 组织培养; 培养基;
Abstract: 本文通过采用基本培养基、诱导培养基和植物激素试验等探索利用菽北种茶外殖体进行快速组织培养的最适培养基.研究发现,用菽北种茶带腋芽的茎段做为外殖体进行组织培养可获得再生植株,并可建立快速无性繁殖系.实验表明,芽增殖最适培养基为1/2MS+BA1.0mg/L+IBA0.2mg/L;生根壮苗最适培养基为1/4MS+BA0.5mg/L+IBA0.1mg/L+A13+0.01mg/L.此外,还探讨了菽北种茶组织培养中褐变问题以及试管苗的移栽.
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