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Determination of Purines in Wheat by Solid Phase Extraction-Reverse Phase High Performance Liquid Chromatography
Author(s): 
Pages: 120-123
Year: Issue:  5
Journal: Journal of Food Science and Biotechnology

Keyword:  啤酒高效液相色谱嘌呤固相萃取;
Abstract: 采用Sep-PakC18 萃取柱,建立了固相萃取-反相高效液相色谱法测定啤酒酿造辅料小麦中的嘌呤类物质含量的方法.该方法准确可靠,重现性好,4种嘌呤的回收率为90.6%~96.4%,相对标准偏差(RSD)小于3%.腺嘌呤(Ade)、鸟嘌呤(Gua)、黄嘌呤(Xan)和次黄嘌呤(Hyp)的最低检测限依次为0.1,0.2,0.1,0.5mg/L.
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