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Construction of Conditional Target-trapping Vector
Author(s): 
Pages: 74-79
Year: Issue:  3
Journal: China Biotechnology

Keyword:  基因捕获突变LoxP条件性基因捕获载体定点基因捕获;
Abstract: 基因捕获是依赖于捕获载体的随机插入产生突变而进行基因功能研究的一类重要技术.由于传统捕获载体在捕获位点上的局限性,各种新型的捕获载体正在不断地被设计并应用于基因捕获技术.以PL-451为基础质粒,结合定点的重组酶识别位点LoxP及Frt序列,突变LoxP的1个碱基的LoxP511A和LoxP511B,SA位点以及不合启动子但有poly(A)的EGFP片断,构建了能够在捕获元件两侧克隆同源臂的务件性基因捕获载体,从而为实现可调控性基因捕获奠定基础.
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