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Effects of deminerazation and platelet-rich plasma on periodontal ligament fibroblasts attachment and proliferation on diseased root surfaces
Author(s): 
Pages: 437-439
Year: Issue:  5
Journal: Journal of Hainan Medical College

Keyword:  富血小板血浆牙周膜成纤维细胞附着增殖;
Abstract: 目的:研究牙周病患牙累及的牙根面经EDTA脱矿和富血小板血浆(PRP)单独及联合处理后对人牙周膜成纤维细胞附着、增殖的影响.方法 :以因牙周病不能保留而拔除的患牙制备的牙根片为对照组,PRP和EDTA脱矿单独及联合处理患牙根片为实验组,用细胞计数法及四甲基偶氮唑盐(MTT)比色实验分析人牙周膜成纤维细胞在病变根面上的附着和增殖.结果:未处理组的根片上有少量的细胞附着;根片经PRP或EDTA脱矿单独处理能明显增加细胞的附着(P<0.05);二者联合处理促附着效果明显增强(P<0.01).未处理组根片上生长的细胞随时间延长数量反而下降;经PRP或EDTA单独处理的根片上的细胞有少量的增殖(P>0.05);二者联合处理细胞增殖明显(P<0.05).结论:EDTA根面脱矿和PRP能有效促进人牙周膜成纤维细胞在牙周病患牙根面上的附着和增殖.
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