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Determin ation of Serum Protein by CPA-Ⅰ Reagent Spectrophotometry
Author(s): 
Pages: 49-52
Year: Issue:  4
Journal: Guangdong Trace Elements Science

Keyword:  偶氮氯膦Ⅰ血清蛋白质分光光度法;
Abstract: 在Na2HPO4 - 柠檬酸酸性缓冲液中,加入非离子表面活性剂聚氧乙烯月桂醚(Brij-35),使偶氮氯膦Ⅰ(CPA - Ⅰ)与牛血清白蛋白形成复合物,复合物的最大吸收波长在570 nm,比试剂本身红移了70 nm,表观摩尔吸光系数为5.88×104 L/(mol·cm 1),线性范围0~166 mg/L,采用分光光度法研究该结合反应的最佳反应条件,并据此建立了一种测定血清蛋白质的新方法.结果表明,该法回收率为98.7%~102.2%,批内变异系数为2.4%,血清用量少,线性范围宽,具有操作快速、简便、结果灵敏可靠等优点,与双缩脲法相关性良好.
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