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Genotype Validation of 12 STR Loci by Sequencing Method
Author(s): LI Cheng-tao, ZHAO Zhen-min, LIU Yan, LI Li
Pages: 115-
117,122
Year: 2009
Issue:
2
Journal: Journal of Forensic Medicine
Keyword: 法医遗传学; 短串联重复序列; 突变;
Abstract: 目的 用测序方法验证12个STR基因座的基因型.方法 根据各STR基因座的特异性序列,对CSF1PO、FGA、TH01、TPOX、VWA、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51和D21S11 12个STR基因座设计了PCR引物并对标准品9947A和突变样本进行PCR产物测序.结果 标准品9947A和突变样本的测序结果均与其基因分型结果一致.结论 建立的测序方法准确、灵敏,可以用于STR基因型的确认.
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