The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit
later.
We apologize for any inconvenience caused
The Application of Three Methods Combined for Extracting DNA from Human Epithelium
Author(s): GUO Yan-xia, CHEN Song, LIU Kai-hui
Pages: 42-
43
Year: 2009
Issue:
1
Journal: Journal of Forensic Medicine
Keyword: 法医遗传学; 提取法; 脱落上皮细胞;
Abstract: 目的 寻求提高法医物证检验中附有人体脱落细胞载体检材的DNA检出率的方法. 方法根据案件检材的具体条件,选择3种提取方法即Chelex-100法、Chelex-100联合有机法和Chelex-100联合磁珠法. 结果采用了分步提取DNA法,可充分、有效地提取微量DNA,提高了人体脱落上皮细胞有效DNA的检出率. 结论根据第一步提取方法的检测结果,来调整下一步的提取策略,如是否加以纯化浓缩,可增加人体脱落上皮细胞有效DNA的检出率.
Citations
No citation found
Related Articles
No related articles found