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Study on qualitative and quantitatire methods for Caratostigma willmottianum
Author(s): 
Pages: 2130-2132
Year: Issue:  17
Journal: China Journal of Chinese Materia Medica

Keyword:  紫金莲薄层色谱白花丹醌高效液相色谱法;
Abstract: 目的:建立紫金莲药材的定性鉴别和定量检测方法.方法:采用TLC法,以紫金莲对照药材和白花丹醌对照品鉴别紫金莲药材;以HPLC测定白花丹醌含量.结果:采用C18色谱柱,以甲醇-水(75∶25)为流动相,检测波长265 nm,供试品色谱中白花丹醌分离良好,白花丹醌进样量在33.6~313.6 ng线性关系良好,r=0.999 9,平均加样回收率100.3%,RSD 1.9%.结论:通过研究制定了紫金莲药材TLC和HPLC质量控制方法.
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