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Cloning,characterization and expression detection of the β-actin cDNA from Mantichorula semenowi(Coleoptera:Tenebrionidae)
Author(s): 
Pages: 1210-1215
Year: Issue:  11
Journal: Acta Entomologica Sinica

Keyword:  谢氏宽漠王β-actin基因同源克隆cDNA快速末端扩增分子内标;
Abstract: β-actin是actin家族的一员,在维持细胞结构、运动和分裂等细胞生理活动方面发挥着重要作用,是基因定量实验中最常用的内参之一.本实验采用同源克隆和RACE技术扩增得到谢氏宽漠王Mantichorula semenowi β-actin基因.序列分析结果表明,该基因cDNA全长1 372 bp,开放阅读框长1 131 bp,编码376个氨基酸,5′和3′末端非翻译区域(UTP)分别为66 bp和175 bp;该序列与其他动物β-actin基因核苷酸序列具有96%~99%高度同源性.β-actin表达量检测结果显示热激后不同恢复时间其表达量无明显变化,且与未经热激处理的对照相比无显著差异.表明β-actin是研究受外界环境胁迫作用下昆虫体内不同基因表达水平的可靠内参基因.
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