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Effects of hypoxia on human placental decidua basalis-mesenchymal stem cells proliferation,apoptosis and VEGF expression
Author(s): 
Pages: 783-789
Year: Issue:  6
Journal: Acta Physiologica Sinica

Keyword:  基蜕膜间充质千细胞低氧增殖凋亡血管内皮细胞生长因子;
Abstract: 本文旨在探讨生理低氧和无血清培养条件下人胎盘基蜕膜间充质干细胞(placental decidua basalis-mesenchymal stem cells,PDB-MSCs)的生物学特征和细胞因子的表达情况.采用密度梯度离心法培养获得PDB-MSCs,利用MTT法、流式细胞术检测PDB-MSCs在不同氧浓度(20%02和1%02)、有无血清00%FBS和0%FBS)条件下各个时间点(6 h、12 h、24h、48 h、72 h、96 h)的增殖和凋亡情况,并采用酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测无血清条件下各个时间点细胞上清液中血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)含量.结果显示,在特定的时间内低氧可以促进PDB.MSCs增殖(P<0.01,n=3),而血清对PDB-MSCs增殖的影响与氧浓度关系密切;同时,在本实验条件下,低氧、无血清分别或联合培养不会导致PDB-MSCs凋亡(P>0.05,n=3);在无血清条件下,24 h时低氧组PDB-MSCs表达较高水平的VEGF.以上结果提示,PDB-MSCs可能成为缺血相关组织工程产品种子细胞的一个新来源.
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