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Overexpression of PDZ 1 domain of PSD-95 protein rescues hippocampl neurons from apoptosis induced by oxygen-glucose deprivation
Author(s): 
Pages: 730-736
Year: Issue:  6
Journal: Acta Physiologica Sinica

Keyword:  PDZ1缺氧缺糖海马神经元凋亡;
Abstract: 为了观察突触后致密物质-95(postsynaptic density 95,PSD-95)结构域PDZ1过表达对缺氧缺糖诱导的海马神经元凋亡的影响,本研究采用培养21 d的Sprague-Dawley大鼠海马神经元,加入PDZ1腺病毒颗粒感染24 h,收集细胞进行免疫沉淀、免疫日J迹实验:缺氧缺糖1.5 h后,用DAPI染色后荧光显微镜观察细胞凋亡,并收集细胞进行免疫印迹实验.结果显示:(1)PDZ1在海马神经元中过表达:(2)过表达的PDZ1使缺氧缺糖诱导的海马神经元凋亡数量减少(P<0.05);(3)过表达的PDZ1使PSD-95与GluR6结合减少;(4)PDZ1过表达抑制由缺氧缺糖诱导的大鼠海马神经元MLK3和JNK1/2磷酸化.以上结果提示,PDZ1过表达能拈抗缺氧缺糖诱导的海马神经元凋亡.
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