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D1-dopamine receptor is involved in the modulation of the respiratory rhythmical discharge activity in the medulla oblongata slice preparation of neonatal rats in vitro
Author(s): 
Pages: 704-708
Year: Issue:  6
Journal: Acta Physiologica Sinica

Keyword:  多巴胺D1受体延髓呼吸节律;
Abstract: 本研究旨在探讨多巴胺D1受体在延髓离体脑片基本节律性呼吸放电调节中的作用.以改良的Kreb'S液(modified Kreb,s perfusion,MKS)恒温灌流Sprague-Dawley大鼠(0~3 d)离体延髓脑片标本,稳定记录到与之相连的舌卜神经根的呼吸节律性放电活动(respiratory rhythmical discharge activity,RRDA)后,第一组(n=5)先给予多巴胺Dl受体特异性激动剂[cis-(±)-1-(Aminomethyl)-3,4-dihydro-3-phenyl-1H-2-Benzopyran-5,6-Diolhy.drochlo-fide,A689301灌流10 min,用MKS洗脱后,再给予多巴胺D1受体特异性拮抗剂[R(+)-7-Chloro-8-hydroxy-3-methyl-1-pheny1-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride,SCH-23390]灌流10 min;第二组(n=5)先给予A68930持续灌流10 min后再给予A68930+SCH-23390持续灌流10 min;观察各时间点舌下神经根RRDA的变化.结果显示,给予A68930灌流后呼吸周期(respiratory cycle,RC)和呼气时程(expiratory time,TE)显著缩短,放屯积分幅度(integral amplitude,IA)增加,吸气时程(inspiratory time,TI)无明显变化;给予SCH-23390后RC、TE显著延长、TI显著缩短、IA减小,而且A68930的作用可以被SCH一23390部分逆转.这些观察结果提示多巴胺D1受体参与了哺乳动物基本呼吸频率和幅度的调节.
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