A study on differentially expressed genes in hepatic stellate cells treated with transforming growth factor beta 1 using cDNA microarray technique
Author(s): XIAO Lin, CHENG Jun, GUO Jiang, HONG Yuan, ZHANG Li-ying, ZHANG Yue-xin, ZHANG Jian-long, LI Yan
Pages: 752-756
Year: 2008 Issue: 10
Journal: CHINESE JOURNAL OF HEPATOLOGY
Keyword: Hepatic fibrosis; Hepatic stellate cell; Transforming growth factor beta; Geneexpression;
Abstract: Objectives To screen the differentially expressed genes in hepatic stellate cells (HSC)treated with transforming growth factor beta 1 (TGF β1) by cDNA microarray technique, and to elucidate the molecular pathogenesis of liver fibrosis involving TGF β 1. Methods Total RNA was extracted from HSC treated with TGF β1 and PBS by trizol and reverse-transcribed to double strand cDNA templates. Transcrip-tion of cDNA probe with biotin-labeling was performed, and then the obtained cDNA was hybridized with human cDNA mieroarray. The results were imaged by an Agilent scanner, and the differentially expressed genes were analyzed with bioinformatics software. Results One hundred seventy-seven differentially ex-pressed genes were screened from 13824 targeting genes; 123 genes were up-regulated, including connective tissue growth factor, tubulin ε 1, collagen, type V, α 2, eatenin δ 2, cadherin 6, type 2, Smad3, mitogen-activated protein kinase 4, growth factor receptor-bound protein 7 and MAP kinase-interacting serine/thren-nine kinase 1; 54 genes were down-regulated, including TNF receptor-associated factor 4, interferon regula-tory factor 7, interferon inducible protein p78, bone morphogenetic protein 7, matrix gla protein, serine proteinase inhibitor, interferon stimulated gene 2.0 × 104, death-associated protein 6, metallothionein 1H and superoxide dismutase 2; in addition, 8 genes with unknown functions were also found. Conclusion The differentially expressed genes in HSC treated with TGF β 1 were successfully screened by cDNA microarray technique. It revealed that the molecular pathogenesis of liver fibrosis involving TGF β 1 was the result of co-regulation by multiple factors. This information might be of help in searching for new targets in gene therapy.
Pages: 752-756
Year: 2008 Issue: 10
Journal: CHINESE JOURNAL OF HEPATOLOGY
Keyword: Hepatic fibrosis; Hepatic stellate cell; Transforming growth factor beta; Geneexpression;
Abstract: Objectives To screen the differentially expressed genes in hepatic stellate cells (HSC)treated with transforming growth factor beta 1 (TGF β1) by cDNA microarray technique, and to elucidate the molecular pathogenesis of liver fibrosis involving TGF β 1. Methods Total RNA was extracted from HSC treated with TGF β1 and PBS by trizol and reverse-transcribed to double strand cDNA templates. Transcrip-tion of cDNA probe with biotin-labeling was performed, and then the obtained cDNA was hybridized with human cDNA mieroarray. The results were imaged by an Agilent scanner, and the differentially expressed genes were analyzed with bioinformatics software. Results One hundred seventy-seven differentially ex-pressed genes were screened from 13824 targeting genes; 123 genes were up-regulated, including connective tissue growth factor, tubulin ε 1, collagen, type V, α 2, eatenin δ 2, cadherin 6, type 2, Smad3, mitogen-activated protein kinase 4, growth factor receptor-bound protein 7 and MAP kinase-interacting serine/thren-nine kinase 1; 54 genes were down-regulated, including TNF receptor-associated factor 4, interferon regula-tory factor 7, interferon inducible protein p78, bone morphogenetic protein 7, matrix gla protein, serine proteinase inhibitor, interferon stimulated gene 2.0 × 104, death-associated protein 6, metallothionein 1H and superoxide dismutase 2; in addition, 8 genes with unknown functions were also found. Conclusion The differentially expressed genes in HSC treated with TGF β 1 were successfully screened by cDNA microarray technique. It revealed that the molecular pathogenesis of liver fibrosis involving TGF β 1 was the result of co-regulation by multiple factors. This information might be of help in searching for new targets in gene therapy.
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