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Effects of "Spleen-Fortifying, Qi-Regulating and Blood-Activating Compound Recipe" on Plasma Endotoxin Content and TNF-α Protein Expression in Alcoholic Liver Injury Rats
Author(s): 
Pages: 44-47
Year: Issue:  5
Journal: ACTA UNIVERSITATIS TRADITIONIS MEDICALIS SINENSIS PHARMACOLOGIAEQUE SHANGHAI

Keyword:  健脾理气活血方酒精性肝损伤内毒素肿瘤坏死因子-α;
Abstract: 目的:观察中药健脾理气活血方对大鼠酒精性肝损伤的防治效果及其血浆内毒素和TNF-α蛋白表达的影响.方法:通过喂饲按Lieber-Decarli配方配制的含酒精液体饲料复制酒精性肝损伤的大鼠模型.32只雄性SD大鼠随机分设正常组(n=5)、无酒精液体饲料组(n=9)、酒精液体饲料组(n=9)及酒精液体饲料+中药组(n=9).在造模第4周开始灌胃给药或蒸馏水.8周末取材观察:①肝组织病理变化(HE和油红染色);②肝组织甘油三酯(TG)含量:③肝损伤指标(血清ALT、AsT活性);④血浆内毒素水平;⑤肝组织P-IKB、TNF-α的蛋白表达(western blot法)的变化.结果:①口服酒精液体饲料8周后,模型大鼠肝细胞出现显著的脂肪变性,肝脏TG含量、血浆内毒素水平以及血清ALT、AST活性均较正常组和无酒精液体饲料组显著升高.此外,肝组织P-IκB、TNF-α蛋白表达增强.②健脾理气活血方组较之模型组,肝组织脂肪变性程度和炎症程度显著减轻,其肝组织TG含量以及血浆内毒素水平显著降低,ALT活性接近正常水平;此外,肝组织P-IKB、TNF-α蛋白表达也较模型组下降.结论:健脾理气活血方具有显著的抗大鼠酒精性肝损伤的作用,其机制与抑制内毒素和大鼠肝组织P-IκB、TNF-α蛋白表达有关.
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