Preparation of soybean phosphatidylethanolamine and phosphatidylinositol by column chromatography
Author(s): JIANG Bo, WAN Jianchun, CONG Yanxia
Pages: 50-52
Year: 2008 Issue: 10
Journal: CHINA OILS AND FATS
Keyword: 大豆油脚; 柱色谱; 制备; 脑磷脂; 肌醇磷脂;
Abstract: 采用溶剂萃取法和柱色谱法相结合的工艺,从大豆油脚中制备肌醇磷脂,研究了不同洗脱剂对柱色谱分离脑磷脂和肌醇磷脂效果的影响.结果表明,大豆油脚先经脱水、脱油,再经95%乙醇浸提后粗分为粗卵磷脂和粗脑磷脂.以硅胶为吸附介质,以粗脑磷脂为原料用V(氯仿):V(甲醇)=2:1的混合溶剂等度洗脱,可获得纯度为80.1%的大豆脑磷脂和90.6%的肌醇磷脂产品.
Pages: 50-52
Year: 2008 Issue: 10
Journal: CHINA OILS AND FATS
Keyword: 大豆油脚; 柱色谱; 制备; 脑磷脂; 肌醇磷脂;
Abstract: 采用溶剂萃取法和柱色谱法相结合的工艺,从大豆油脚中制备肌醇磷脂,研究了不同洗脱剂对柱色谱分离脑磷脂和肌醇磷脂效果的影响.结果表明,大豆油脚先经脱水、脱油,再经95%乙醇浸提后粗分为粗卵磷脂和粗脑磷脂.以硅胶为吸附介质,以粗脑磷脂为原料用V(氯仿):V(甲醇)=2:1的混合溶剂等度洗脱,可获得纯度为80.1%的大豆脑磷脂和90.6%的肌醇磷脂产品.
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