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Establishment of highly regeneration system in vitro of protocorn-like body thin cell layers of Cymbidium hybridum
Author(s): 
Pages: 313-317
Year: Issue:  5
Journal: ACTA AGRICULTURAE ZHEJIANGENSIS

Keyword:  大花蕙兰类原球茎薄层切片离体诱导;
Abstract: 通过对大花蕙兰离体诱导类原球茎几个主要影响因素的研究,构建优良、高效的离体诱导体系.研究表明,不同外植体类型再生能力有显著差异,其中以类原球茎(protocorn-like body,PLB)横切片(transverse thin cell layers,tTCLs)为外植体可获得最高的PLB再生频率,叶片再生频率及数量最低.不同激素配比影响tTCLs的再生能力,其中在1/2 MS+TDZ 1.0 mg/L +KT 0.5 mg/L +NAA 0.5 mg/L上最高诱导频率为91.20%.在上述培养基中添加3.0 mg/L AgNO3可提高tTCLs上PLBs的形成,但作用不显著.可见,以tTCLs为外植体诱导大花蕙兰PLBs切实可行.
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