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Cloning of aerolysin gene of Aeromonas trota from Eriocheir sinensis and rapid detection by PCR
Author(s): 
Pages: 344-348
Year: Issue:  5
Journal: ACTA AGRICULTURAE ZHEJIANGENSIS

Keyword:  易损气单胞菌气溶素基因PCR;
Abstract: 利用设计的特异引物,采用PCR技术,对蟹源致病性易损气单胞菌气溶素基因(aer)进了克隆和序列分析,从其基因组中克隆出长为622 bp的基因片断,用限制性内切酶BamHI对扩增产物进行酶切,产生长约570 bp和50 bp两条片断;研究还利用设计的特异引物,对蟹源致病性易损气单胞菌进行PCR快速分子鉴定的研究.
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