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A New Automatic Method for Analysis of TIBC in Serum with Three Reagents:Nitro-PAPS Direct Spectrophotometric Method
Author(s): 
Pages: 30-33
Year: Issue:  9
Journal: GUANGDONG TRACE ELEMENTS SCIENCE

Keyword:  总铁结合力血清自动分析法Nitro-PAPS;
Abstract: 为建立简便、灵敏的三试剂自动化分析法测定血清总铁结合力(TIBC),在表面活性剂存在下,用2-(5-硝基-2 - 吡啶偶氮)-5 - [N-正丙基-N - (3-磺酸丙基)氨基]苯酚二钠(Nitro-PAPS)为显色剂测定了血清TIBC.结果表明,该法线性范围0~195 μmol/L,平均回收率为100.7%,批内变异系数(CV)和批间变异系数分别为0.019、0.032;与临床检验操作规程推荐的方法比较具有良好的相关性,线性回归方程和相关系数分别为y =1.002 1 x+1.210 0,r=0.979 8;78例健康男性血清TIBC含量(x±2s)为46.8~76.1 μmol/L,75例健康女性为52.8~77.6 μmol/L.方法简便、灵敏可靠,适合临床应用.
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