Differentiation effecs on DNA polymerase beta and X-ray repair cross-complementing protein 1 expression in Eca 109 cells
Author(s): LI Shi-kun, XU Jun, CHEN Ke-he, REN Qing-mei
Pages: 1267-1268
Year: 2008 Issue: 8
Journal: CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
Keyword: DNA polymerase beta; Eca-109 cell line; X-ray repair cross-complementing protein 1;
Abstract: Objective To study the change of DNA polymerase beta and XRCC1's expression during malignant celI differentiation.Methods The Eca-109 cells were divided inm 2 groups:differentiation group which cultured with 8-Br-cAMP and control group.The 2 groups cells were cultured 48h simultaneously.The immunocytochemistry was performed to detect the expression of DNA polymerase beta and XRCC1.Results Compared with control group,the expression of DNA polymerase beta and XRCC1 was decreased simultaneously(P＜0.05).Conclusion The differentiation agent can down-regulate the expression of DNA polymerase beta and XRCC1,suggesting that overexpressed DNA polymerase beta and XRCC1 maybe result in mutator phenotype.
Pages: 1267-1268
Year: 2008 Issue: 8
Journal: CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
Keyword: DNA polymerase beta; Eca-109 cell line; X-ray repair cross-complementing protein 1;
Abstract: Objective To study the change of DNA polymerase beta and XRCC1's expression during malignant celI differentiation.Methods The Eca-109 cells were divided inm 2 groups:differentiation group which cultured with 8-Br-cAMP and control group.The 2 groups cells were cultured 48h simultaneously.The immunocytochemistry was performed to detect the expression of DNA polymerase beta and XRCC1.Results Compared with control group,the expression of DNA polymerase beta and XRCC1 was decreased simultaneously(P＜0.05).Conclusion The differentiation agent can down-regulate the expression of DNA polymerase beta and XRCC1,suggesting that overexpressed DNA polymerase beta and XRCC1 maybe result in mutator phenotype.
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