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CD34+ cells and T cell subsets in recombinant human granulocyte colony-stimulating factor primed bone marrow grafts from donors with different characteristics
Pages: 512-516
Year: Issue:  8

Keyword:  Granalocyte colony-stimulating factorDonor characteristicsBone marrow graftsCD34+ cellsT-lymphocyte subsets;
Abstract: Objective To investigate the cell composition in granulocyte colony-stimulating factor (rhG-CSF)primed bone marrow grafts(G-BM)from donors with different characteristics.Methods One tive days.Bone marrow and peripheral blood stem cells were harvested at day 4 and day 5.The number of CD3+,CD3+ CD4+,CD3+ CD8+,CD14+,CD34+ and CD3+ CD4-CD8-cells were determined by multicolor flow cytometry.Cell composition of G-BM from donors with different characteristics,including sex,age,weight,pregnancy were analysed.Results The absolute number of nuclear cells(NCs),lymphocytes,CD3+,CD3+ CD4+ ,CD3+ CD8+,CD14+,CD34+ and CD3+ CD4-CD8-cells in G-BM were 31 050 (12 200-58 100),2122(506-6618),1344(307-4791),692(145-3038),616(112-2575),986 (265-2958),63(11-505)and 83(9-390)per microliter,respectively.The number of NCs[33 800 (18 600-57 100)],CD134+ cells[76(22-505)],and CD3+ CD4-CD8-regulatory T cells[97(11-380)]harvested from younger donom(aged≤38 years)were significantly higher than those from older ones (aged>38)[28 000(12 200-58 100),49(11-220),and 65(9-389)per microliter(P=0.027,<0.001 and=0.001,respectively)].Compared with that in donors with peripheral blood monocytes (PBMs)≤0.37×109 /L,higher number of NCs in G-BM[27 150(13 800-58 100) vs 33 550(12 200-57 100),P=0.005 ] were collected in donors with PBMs>0.37×109 /L.Multivariate analysis showed that donors age(≤38 vs >38 years;P=0.01)and monocyte number in peripheral blood(≤0.37×109 /L vs >0.37×109 /L;P=0.003)were factors predicting for NC yields,and donors age(≤38 vs >38 years;P<0.001)was factor predicting for yields of CD34+ cells(P<0.001)and CD3+ CD4-CD8- regulatory T cells (P=0.001)collection.Conclusion Donor age is a factor for the yields of NCs,CD34+ cells,and CD3+ CD4-CD8-regulatory T cells collection in G-BM,and donor's PBMs more than 0.37×109 /L,is another factor affecting NCs harvests.
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