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Experimental study on influence of bone marrow mesenchymal stem cells on activation and function of mouse peritoneal macrophages
Author(s): 
Pages: 540-543
Year: Issue:  8
Journal: CHINESE JOURNAL OF HEMATOLOGY

Keyword:  Mesenchymal stem cellsMouse peritoneal macrophagesActivationLipopolysarchride;
Abstract: Objective To explore the influence of bone marrow(BM)mesenchymal stem cells (MSCs)on macrophage activation after ipopolysarchride(LPS)stimulation.Methods Mouse BM MSCs were isolated and purified by adherence screening,and mouse peritoneal macrophages(MPM)were collected by sodium thioglycollate peritoneal injection,and the co-culture system was established by planting macrophages on the MSCs monolayer.The grouping of experiments:group A:MPM;group B:MPM+LPS;group C:MPM+LPS+MSC;group D:MPM+LPS+MSC supernatant.Cell culture supematants were collected to detect the changes of TNF-α/TGF-β and nitrogen monoxide(NO)after stimulating macrophages with LPS for 18 hours.At the same time Escbericbia coli standard strain(ATCC25922)was added into the culture system and incubated for another 24 hours,macrophages were stained and phagocytoses were examined.Results The concentrations of TNF-α and NO in culture supernatants were increased significantly to(147.4±37.1)Pg/ml and(59.9±8.7)μmol/L respectively after macrophage activation,however,at the present of MSC,the concentration of TNF-α was dramatically decreased[(97.6 ±30.3)pg/ml,P=0.032],and the concentration of NO was decreased to(50.9±29.5)μmol/L(P>0.05).The concentrations of TNF-α and NO were further decreased after addition of MSC supematants[(58.3±31.5)pg/ml and(-3.4±2.3)μmoL/L respectively,P<0.01].There was no change in the phagocytic rate and phagoindex of macrophages after activation.Conclusions MSCs can inhibit the activation of mouse peritoneal macrophages after stimulating with LPS but has no influence on the phagocytosis.
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