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The Phenomenon and Preventing of Vitrification in Tissue Culture of Dianthus cargophyllus
Author(s): ZHANG Hong, WANG Wan-xin
Pages: 196-
197
Year: 2008
Issue:
8
Journal: NORTHERN HORTICULTURE
Keyword: 香石竹; 组织培养; 玻璃化;
Abstract: 玻璃化是香石竹组织培养中的一大障碍,试验研究了BA、琼脂、蔗糖、活性炭等因素对香石竹组培苗玻璃化的影响,结果表明:降低细胞分裂素的浓度,提高琼脂的用量,培养基中加入0.5g/L活性炭对克服香石竹组培苗玻璃化有明显的效果;增加蔗糖的用量对香石竹玻璃化的防止没有明显的作用;香石竹组织培养中能有效防止玻璃化的适宜培养基为MS+BA 0.5mg/L+NAA 0.01mg/L+蔗糖30g/L+琼脂8g/L+活性炭0.5g/L.
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