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MYCOBACTERIOPHAGE BASED ASSAY ON RIFAMPIN SUSCEPTIBILITY TESTING OF MYCOBACTERIUM TUBERCULOSIS
Author(s): 
Pages: 963-965
Year: Issue:  7
Journal: CHINESE JOURNAL OF COAL INDUSTRY MEDICINE

Keyword:  结核分枝杆菌药物敏感性试验分枝杆菌噬菌体;
Abstract: 目的 探讨噬菌体法用于检测结核分枝杆菌利福乎耐药性的应用价值.方法 选取148株结核分枝杆菌临床分离株,用噬菌体法和改良L-J培养基绝对浓度法同时进行利福平耐药性检测.结果 绝对浓度法结果 表明,148株中有98株为利福平耐药,50株敏感.耐药的98株中,噬菌体法检出了96株,50株敏感株中噬菌体法的结果 全为阴性.噬菌体法与常规的L-J培养基绝对浓度法取得了较高的符合率,噬菌体法的敏感性为98%,特异性为100%.结论 噬菌体法检测结核分枝杆菌利福乎耐药性,操作简便,快速,敏感.特异,在48~72h内可得出结果 ,具有良好的临床应用前景.
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