Comparison of rabbit model of degenerative intervertebral disc by anulus puncture and nucleus aspiration
Author(s): Zhao Xin, Zhao Xingang, Zhang Hailong, Huang Shi, Liu Chaoqian, Hou Tiesheng
Pages: 394-396
Year: 2008 Issue: 3
Journal: CHINESE JOURNAL OF ANATOMY
Keyword: 纤维环穿刺法; 髓核抽吸法; 椎间盘退变; 动物模型;
Abstract: 目的:比较采用纤维环穿刺法和髓核抽吸法建立兔椎间盘退变动物模型的不同.方法:新西兰大白兔经腹膜外入路暴露腰3/4、腰4/5、腰5/6椎间隙,纤维环穿刺组采取针刺纤维环,髓核抽吸组抽吸髓核8周后核磁共振观察椎间盘变化,生化分析椎间盘中蛋白多糖含量,免疫组织化学检测Ⅱ型胶原表达.结果:术后8周与对照组相比,采用两种方法的椎间隙高度与T2信号手术前后变化比值,以及Ⅱ型胶原和蛋白多糖含量,差异显著.其中髓核抽吸组的Ⅱ型胶原和蛋白多糖含量明显低于纤维环穿刺组.结论:髓核抽吸法建立兔退变椎间盘模型退变程度大于纤维环穿刺法.
Pages: 394-396
Year: 2008 Issue: 3
Journal: CHINESE JOURNAL OF ANATOMY
Keyword: 纤维环穿刺法; 髓核抽吸法; 椎间盘退变; 动物模型;
Abstract: 目的:比较采用纤维环穿刺法和髓核抽吸法建立兔椎间盘退变动物模型的不同.方法:新西兰大白兔经腹膜外入路暴露腰3/4、腰4/5、腰5/6椎间隙,纤维环穿刺组采取针刺纤维环,髓核抽吸组抽吸髓核8周后核磁共振观察椎间盘变化,生化分析椎间盘中蛋白多糖含量,免疫组织化学检测Ⅱ型胶原表达.结果:术后8周与对照组相比,采用两种方法的椎间隙高度与T2信号手术前后变化比值,以及Ⅱ型胶原和蛋白多糖含量,差异显著.其中髓核抽吸组的Ⅱ型胶原和蛋白多糖含量明显低于纤维环穿刺组.结论:髓核抽吸法建立兔退变椎间盘模型退变程度大于纤维环穿刺法.
Citations
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